Ba‐Wei‐Di‐Huang‐Wan through its active ingredient loganin counteracts substance P‐enhanced NF‐κB/ICAM‐1 signaling in rats with bladder hyperactivity

摘要: Overt bladder afferent activation may exacerbate endogenous substance P (SP) release to induce intercellular adhesion molecule-1 (ICAM-1)-mediated inflammation and reactive oxygen species (ROS) production leading hyperactive bladder. Ba-Wei-Die-Huang-Wan (BWDHW), a traditional Chinese medicine, has been used treat lower urinary tract symptoms in patients by undefined mechanisms. We explored the possible mechanisms active components of BWDHW on exogenous SP-induced hyperactivity. contained six major components: loganin, paeoniflorin, 5-hydroxymethylfurfural, cinnamic acid, cinnamaldehyde, paeonol high-performance liquid chromatography. In urethane-anesthetized female Wistar rats, we evaluated transcystometrogram, pelvic nerve activity electrophysiologic recording techniques, ICAM-1 expression Western blot immunohistochemistry, ROS amount an ultrasensitive chemiluminescence method sources from different leukocytes specific stains SP-treated its component loganin dose-dependently inhibited H2 O2 HOCl vitro. Intragastrical (250 mg/kg) (5 mg/kg) twice daily for 2 weeks did not affect baseline micturition parameters. Intra-arterial SP (20 µg/rat) through neurokinin-1 receptor increased voiding frequency (shortened intercontraction intervals), activity, NF-κB/ICAM-1 expression, amount, neutrophils venous endothelium, CD68 (monocyte/macrophage), mast cell infiltration inflamed pretreatment significantly depressed SP-enhanced leukocyte infiltration, subsequently improved conclusion, our results suggest that improves hyperactivity via inhibiting SP/neurokinin-1 signaling depressing mediated oxidative injury inflammation. Neurourol. Urodynam. 35:771-779, 2016. © 2015 Wiley Periodicals, Inc.