Innate and adaptive immunity gene expression of human keratinocytes cultured of severe burn injury
作者： Silvana Aparecida Alves Corrêa de Noronha , Samuel Marcos Ribeiro de Noronha , Larissa Elias Lanziani , Lydia Masako Ferreira , Alfredo Gragnani
DOI: 10.1590/S0102-86502014001700012
关键词: Gene expression 、 CD86 、 Gene 、 Interleukin 8 、 Dispase 、 Acquired immune system 、 Molecular biology 、 Immunology 、 Complementary DNA 、 Human leukocyte antigen 、 Biology
摘要: PURPOSE: Evaluate the expression profile of genes related to Innate and Adaptive Immune System (IAIS) human Primary Epidermal keratinocytes (hPEKP) patients with severe burns. METHODS: After obtaining viable fragments skin without burning, culture hKEP was initiated by enzymatic method using Dispase (Sigma-Aldrich). These cells were treated Trizol(r) (Life Technologies) for extraction total RNA. This quantified analyzed purity cDNA analysis gene specific IAIS PCR Arrays plates (SA Biosciences). RESULTS: we found that 63% these differentially expressed, which 77% repressed 23% hyper-regulated. Among these, following (fold increase or decrease): IL8 (41), IL6 (32), TNF (-92), HLA-E (-86), LYS (-74), CCR6 (- 73), CD86 (-41) HLA-A (-35). CONCLUSIONS: study contributes understanding molecular mechanisms underlying wound infection caused burn. Furthermore, it may provide new strategies restore normal thereby change healing process improve clinical outcome.
fapesp.br
sci-hub.st 参考文章(34)
Laura Campbell, Helen Williams, Rachel A Crompton, Sheena M Cruickshank, Matthew J Hardman, Nod2 deficiency impairs inflammatory and epithelial aspects of the cutaneous wound-healing response. The Journal of Pathology. ,vol. 229, pp. 121- 131 ,(2013) , 10.1002/PATH.4095
Silvia Gariboldi, Marco Palazzo, Laura Zanobbio, Silvia Selleri, Michele Sommariva, Lucia Sfondrini, Stefano Cavicchini, Andrea Balsari, Cristiano Rumio, Low Molecular Weight Hyaluronic Acid Increases the Self-Defense of Skin Epithelium by Induction of β-Defensin 2 via TLR2 and TLR4 Journal of Immunology. ,vol. 181, pp. 2103- 2110 ,(2008) , 10.4049/JIMMUNOL.181.3.2103
Gerd G. Gauglitz, Siegfried Zedler, Felix v. Spiegel, Jasmin Fuhr, Guido Henkel v. Donnersmarck, Eugen Faist, Functional Characterization of Cultured Keratinocytes after Acute Cutaneous Burn Injury PLoS ONE. ,vol. 7, pp. e29942- ,(2012) , 10.1371/JOURNAL.PONE.0029942
Mittra Eren, Mario Fabri, Thomas Krieg, Sabine A. Eming, Pustulosis acuta generalisata with joint involvement in an HLA-A2– and HLA-B35–positive patient Journal of the American Academy of Dermatology. ,vol. 58, pp. 1056- 1058 ,(2008) , 10.1016/J.JAAD.2008.02.034
Alfredo Gragnani, Bruno Rafael Müller, Ismael Dale Contrim Guerreiro da Silva, Samuel Marcos Ribeiro de Noronha, Lydia Masako Ferreira, Keratinocyte growth factor, tumor necrosis factor-alpha and interleukin-1 beta gene expression in cultured fibroblasts and keratinocytes from burned patients Acta Cirurgica Brasileira. ,vol. 28, pp. 551- 558 ,(2013) , 10.1590/S0102-86502013000800001
Ying-yi Luan, Yong-ming Yao, Lei Zhang, Ning Dong, Qing-hong Zhang, Yan Yu, Zhi-yong Sheng, Expression of tumor necrosis factor-α induced protein 8 like-2 contributes to the immunosuppressive property of CD4(+)CD25(+) regulatory T cells in mice. Molecular Immunology. ,vol. 49, pp. 219- 226 ,(2011) , 10.1016/J.MOLIMM.2011.08.016
Jennifer R. Sasaki, Qiong Zhang, Martin G. Schwacha, Burn Induces a Th-17 Inflammatory Response at the Injury Site Burns. ,vol. 37, pp. 646- 651 ,(2011) , 10.1016/J.BURNS.2011.01.028
Jihong Shi, Jun Li, Hao Guan, Weixia Cai, Xiaozhi Bai, Xiaobing Fang, Xiaolong Hu, Yaojun Wang, Hongtao Wang, Zhao Zheng, Linlin Su, Dahai Hu, Xiongxiang Zhu, Anti-Fibrotic Actions of Interleukin-10 against Hypertrophic Scarring by Activation of PI3K/AKT and STAT3 Signaling Pathways in Scar-Forming Fibroblasts PLoS ONE. ,vol. 9, pp. e98228- ,(2014) , 10.1371/JOURNAL.PONE.0098228
N.S. Gibran, M. Ferguson, D.M. Heimbach, F.F. Isik, Monocyte chemoattractant protein-1 mRNA expression in the human burn wound. Journal of Surgical Research. ,vol. 70, pp. 1- 6 ,(1997) , 10.1006/JSRE.1997.5017
Andrew D Luster, Ronen Alon, Ulrich H von Andrian, Immune cell migration in inflammation: present and future therapeutic targets Nature Immunology. ,vol. 6, pp. 1182- 1190 ,(2005) , 10.1038/NI1275