Genetic variability of the invasive cyanobacteria Cylindrospermopsis raciborskii from Bir M'cherga reservoir (Tunisia)

摘要: Cylindrospermopsis raciborskii is an invasive freshwater cyanobacteria of tropical origin, also found in temperate regions. Due to its known ability produce potent toxins, such as cylindrospermopsin and the paralytic shellfish poisoning, this species major concern from a water quality perspective. This study presents genetic characterization four C. strains isolated Bir M’cherga Tunisian reservoir. The toxicity assessment was investigated via molecular biology tools, which suggested that all were not producing cylindrospermopsin, saxitoxin, or microcystin. result further confirmed by HPLC MALDI-TOF analyses. However, we report for first time presence mcyA mcyE, two segments microcystin synthetase mcy cluster. All identified taxonomically based on 16S rRNA sequences, their phylogenetic relationships assessed using rpoC1 region. formed distinct clade separated other African strains.