Complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain

摘要: Tomato spotted wilt virus (TSWV) occurs worldwide and causes production losses in many important horticultural crops such as tomato pepper. Breeding resistant cultivars has been the most successful method so far for TSWV disease control, but only two genes have found to confer resistance against a wide spectrum of isolates: Sw-5 Tsw However, resistance-breaking isolates emerged different countries few years after using cultivars. In this paper, we report first complete nucleotide sequences three Spanish with biotypes according their abilities overcome resistance: LL-N.05 (wild type, WT), Pujol1TL3 (Sw-5 breaking, SBR) PVR (Tsw resistance-breaking, TBR). The genome these consisted segments: L (8913-8914 nt), M (4752-4825 nt) (S 2924-2961 nt). Variations genomic RNA lengths among are reported here. Phylogenetic analysis five open reading frames showed evidence reassortment between segments Pujol1TL3, which was supported by recombination-detecting algorithms.