The expression of insulin-like growth factors and their binding proteins in normal human lymphocytes

摘要: The expression of insulin-like growth factors and their binding proteins in normal human peripheral lymphocytes was studied using the reverse transcriptase polymerase chain reaction method Western ligand blotting. A quantitation RT-PCR products used to study differences between PHA stimulated lymphocytes. Normal freshly collected expressed mRNAs for both IGF-I receptor IGF-II but no corresponding detectable. After stimulation with phytohemagglutinin lymphocytes, however, IGF-II. Of five IGFBPs examined, unstimulated only IGFBP-2 -3. Stimulated IGFBP-4 -5, addition -3, whereas IGFBP-1 mRNA remained undetectable. blotting lymphocyte conditioned media revealed production 34K, 43K 49K IGFBPs. estrogen, progesterone, or hormone did not affect secretion by