Nuclear ratios and complementation in common-A heterokaryons of Schizophyllum commune.

摘要: Seed germination of Melampyrum lineare Desr. requires activation (20 C storage or treatment with gibberellic acid) followed by an extended period exposure to cold. Without these conditions seeds remain dormant. structure and pattern development following are described. Aseptic culture embryos excised from dormant showed growth be possible on agar containing glucose but not potato starch. Embryos gibberellin-activated growing gibberellin grew at the same rate could grow Thus gibberellin-sensitive dormancy mechanism does involve synthesis in embryo starch-digesting enzymes. This mean that such a is located endosperm Melamnpyrum different described for barley oats OUR PREVIOUS investigations (Curtis CantIon, 1963; Cantlon et al., 1963) Desr., chlorophyllous, hemiparasitic angiosperm, have unusual pattern. Germination proceeds only after cold (4 C). In laboratory activated either higher temperatures C) which activates up 40%Jo seed batch, (GA) 20 100% seeds. After (6-10 weeks), germinate Remloval temperature before this prevents germlination. Under field apparently achieved normal (> 15-40 Up 40% immediately set remainder subsequent summers. Michigan populations some viable normally carries over litter least into third year. A as involving two separate temperaturedependent steps exogenous GA acting specifically one them unique. We therefore thought plant might prove 1 Received publication August 10, 1964. work was supported grant GB-1220 National Science Foundation. useful experimental subject study process role GA. The present paper presents data thesequence events leading theintact preliminary studies ow embryos. A\IATERIALS AND METHODS Seeds were collected late summer 1963 natural parasite jack pine forest Crawford County, 1\lichigan. stored followingcollection, 6-8 weeks ca. 15% germinated result prior col-lection. remaining nonactivated re-mained used 6 months storage. soaking 2000 ppm 24 hr washing running tap water 5 muin. procedure re-sults Gibberellin purchased Nutritional Biochemical Co. preparation 75% potassium salt acid. Solutions prepared byadding minimal quantity hydroxidesolution dissolve subsequently neutralizing hydrochloric For aseptic embryos, soaked 1% sodium hypochlorite solution then washed sterile distilled water.. content downloaded 157.55.39.92 Wed, 22 Jun 2016 07:07:08 UTC All use http://about.jstor.org/terms