Inhibition of platelet spreading from plasma onto glass by an adsorbed layer of a novel fluorescent‐labeled poly(ethylene oxide)/poly(butylene oxide) block copolymer: Characteristics of the exclusion zone probed by means of polystyrene beads and macromolecules

摘要: We have investigated the anti-adhesive properties of a newly synthesized fluorescent triblock copolymer containing poly(ethylene oxide). This adsorbs from aqueous solution onto glass that has been rendered hydrophobic. When polymer-treated surface was exposed to human platelet-rich plasma (PRP) or whole blood at 37°C, platelet adhesion and spreading were prevented. Avid rapid occurred along tracks scraped in adsorbed polymer coating, as seen by video-enhanced interference reflection microscopy. Leukocytes are eventually able adhere remove labeled their vicinity accumulate it cell body. Interferometry using polystyrene spheres showed they do not polymer-coated unable approach closer than 70–95 nm. On tracks, beads make molecular contacts with glass. Because fully extended solvated (EO)400 arms may extend up 100 nm glass, this suggests forms monolayer hydrophilic projecting into water, whereas hydrophobic (BO)55 segment binds molecule surface. Another tribloc shorter allows particles more closely. © 1994 John Wiley & Sons, Inc.