Ozone-induced dissociation on a traveling wave high-resolution mass spectrometer for determination of double-bond position in lipids.

摘要: Rationale The position of C=C within fatty acyl chains affects the biological function lipids. Ozone induced dissociation-mass spectrometry (OzID-MS) has great potential in determination lipid double bond position, but only been implemented on low resolution ion trap mass spectrometers. In addition, most OzID-MS experiments carried out so far were focused sodiated adducts lipids, fragmentation commonly observed protonated ions generated LC-MS based lipidomics workflow less explored. Methods Ozone line from an ozone generator was connected to and transfer gas supply a Synapt G2 high spectrometer. Protonated different phosphatidylcholines (PC) by electrospray ionization through direct infusion. Different parameters, including traveling wave height velocity, entrance DC adjusted maximize OzID efficiency. sn- positional isomers cis/trans lipids compared for their reactivity with ozone. Results Traveling Wave velocity tuned prolong encounter time between ozone, resulted improved efficiency, as did increasing trapping region potential. Under optimized settings, at least 1000 times enhancement efficiency achieved that under default settings monounsaturated PC standards. Monounsaturated sn-2 isomer reacted faster than sn-1 isomer. Similarly, trans cis PC. Conclusions This is first implementation enabled The reaction significantly slowing down prolonged interaction ozone. This will facilitate application lipidomics.