Polymers containing enzymatically degradable bonds, 1. Chymotrypsin catalyzed hydrolysis of p‐nitroanilides of phenylalanine and tyrosine attached to side‐chains of copolymers of N‐(2‐hydroxypropyl)methacrylamide

摘要: A series of copolymers N-(2-hydroxypropyl)methacrylamide were prepared, which contained side chains the general formula -Gly-X-Y-NAp, where Gly ¨ glycine; X glycine, alanine, β-alanine, valine, leucine, isoleucine, phenylalanine; Y phenylalanine or tyrosine; NAp p-nitroanilide, latter modelling biologically active compounds. The rates chymotrypsin-catalyzed hydrolysis p-nitroanilide groups at pH = 8,0 and 25°C determined over a range substrate concentrations to derive values for kcat KM. results allowed us determine influence structure on rate cleavage Y-NAp. increase in susceptibility chymotrypsin attack with an increasing spacing Y-NAp residue from backbone polymer is demonstrated by comparing kinetic data containing -Gly-Gly-Phe-Phe-NAp, -Gly-Gly-Phe-NAp -Gly-Phe-NAp chains. Results obtained α-chymotrypsin compared above substrates covalently bound copolymer N-(2-hydroxypropyl)methacrylamide.