Taxol interferes with the interaction of microtubule-associated proteins with microtubules in cultured neurons

作者: MM Black

DOI: 10.1523/JNEUROSCI.07-11-03695.1987

关键词: Cell biologyPaclitaxelPodophyllotoxinCell cultureNeuronBiologyMicrotubule-associated proteinCytoskeletonTubulinMicrotubule

摘要: Treatment of neurons with taxol leads to the formation microtubule bundles in which individual microtubules are much more closely spaced than untreated (Letourneau and Ressler, 1984). This suggests that interferes mechanisms regulate spacing situ. I have determined whether treatment alters composition their microtubules, reasoning such alterations may be related taxol-induced spacing. Cultures sympathetic were incubated 35S-methionine either taxol, podophyllotoxin, a potent microtubule-depolymerizing agent, or dimethyl sulfoxide (DMSO), solvent for drugs. The levels labeled microtubule-associated proteins (MAPs) assembled into various cultures then assayed biochemically. focused on 4 MAPs: tau, chartins, MAP-2, MAP molecular mass 210,000 Da (210 kDa). In cultures, these MAPs prominent components microtubules. all MAPs, as well tubulin, greatly reduced treated compared controls. Taxol had varied effects interaction Microtubules formed presence contained normal slightly elevated tau 210 kDa control cultures. contrast, almost completely devoid chartin MAP-2 These results show some, but not all, altered taxol-treated contribute abnormal seen neurons.

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