Cytoskeletal and morphological alterations underlying axonal sprouting after localized transection of cortical neuron axons in vitro.

摘要: We examined the cytoskeletal dynamics that characterize neurite sprouting after axonal injury to cortical neurons maintained in culture for several weeks and compared these with initial development. Cultured neocortical neurons, derived from embryonic day 18 rats, were at 3 d vitro (DIV) various time points axotomy 21 DIV. The postinjury neuritic response was highly dynamic, progressing through an phase of retraction, followed by substantial within 4-6 hr. Postinjury sprouts motile slender expanded growth cone-like end structures. Microtubule markers localized sprout shafts proximal regions putative cones filamentous actin distributed throughout cones, whereas neurofilament proteins restricted shafts. A similar distribution present developing neurites Exposure mature, injured cultures microtubule stabilizing agent taxol (10 microg/ml) caused inhibition, process distension, transformation into bulbous structures, abnormal directionality. segregation occurred exposure sprouts. destabilizing nocodazole (100 resulted morphological alteration inhibited sprouting. Our results indicate axons have intrinsic ability transection, underlie development