Axonal shearing in mature cortical neurons induces attempted regeneration and the reestablishment of neurite polarity

摘要: While functional recovery after injury is limited, it has become evident that the mature central nervous system does retain some ability to regenerate. This study investigated intrinsic capacity of relatively cortical neurons (21 days in vitro) respond axonal loss. Neurons, growing as clusters on poly-l-lysine, were completely sheared axons through chemical and mechanical disruption transferred either an intact astrocyte monolayer or a substrate poly-l-lysine. Injured exhibited regenerative sprouting response was independent neuronal cell division neural progenitors, demonstrated by negative bromodeoxyuridine (BrdU) precursor intermediate filament nestin, labeling. At 24 h injury, had extended appropriately polarized neurites, compartmentalized microtubule-associated proteins MAP2 tau immunolabeling. Newly tipped growth cones; however, cones tips (mean area, 26.32 ± 2.20 μm) significantly (p < 0.05) smaller than their developmental counterparts 48.64 5.9 μm), substrate. Furthermore, live imaging indicated regenerating distinct dynamics, with significant reduction (70%) pausing, considered vital for interstitial branching pathfinding, relative cones. indicates cultured pyramidal interneurons have potential survive, extend processes, reestablish neurite polarity following physical damage. These results may aid defining cellular basis structural plasticity role reactivity trauma.