Mild axonal stretch injury in vitro induces a progressive series of neurofilament alterations ultimately leading to delayed axotomy.

摘要: We report a new model of transient axonal stretch injury involving pressurized fluid deflection bundles axons, resulting in 1–6% increase original axon length to investigate the slow progression alterations that are characteristic diffuse (DAI). found no discernable difference bundle morphology or cytoskeletal neurofilament protein arrangement between unstretched and stretched at 24 h post-injury. However, by 48 post-injury, there was stereotypical response axons bear similarities vivo neuronal responses associated with DAI have been reported previously. For instance, immunoreactivity (SMI-312) increased contained within 51% all injured compared surrounding bundles, suggestive compaction. Furthermore, derangement occurred 25% ax...