The use of Lucifer Yellow and Mini-Ruby for intracellular staining in fixed brain tissue: methodological considerations evaluated in rat and human autopsy brains
作者： Thomas G. Ohm , Silvia Diekmann
DOI: 10.1016/0165-0270(94)90046-9
关键词: Human brain 、 Intracellular staining 、 Postmortem studies 、 Intracellular 、 Autopsy 、 Central nervous system 、 Lipofuscin 、 Pathology 、 Biology 、 Lucifer yellow
摘要: Abstract The quality of intracellular filling Mini-Ruby (MR) is comparable to that Lucifer Yellow (LY) in both perfusion-fixed rat and immersion-fixed autopsy human tissue. In adult brain, the use MR avoids co-conversion typical lipofuscin deposits as invariably case during photoconversion LY. studies ( n = 35), staining does not correlate with age (range: 25–91 years) or postmortem delay 7–50 h). A short agonal status, however, advantageous.
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