New insight into the surface denaturation of proteins: electronic sum frequency generation study of cytochrome c at water interfaces.
作者： Pratik Sen , Shoichi Yamaguchi , Tahei Tahara
DOI: 10.1021/JP8061288
关键词: In situ 、 Crystallography 、 Spectral line 、 Chemistry 、 Sum-frequency generation 、 Denaturation (biochemistry) 、 Sum frequency generation spectroscopy 、 Cytochrome c 、 Analytical chemistry 、 A protein 、 Bulk water
摘要: In situ characterization of surface denaturation a protein was realized by newly developed interface-selective multiplex electronic sum frequency generation spectroscopy. The observed spectra cytochrome c at the air/water interface exhibited broad feature, which demonstrated coexistence nativelike and denatured interface. This situation mixed conformation did not change in acidic condition pH = 2 where completely bulk water. sharp contrast, only native spectrum silica/water
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