Strategies for isolating somatic cell mutants defective in lipid biosynthesis.

摘要: Publisher Summary This chapter describes three techniques for isolating somatic cell mutants defective in phospholipid biosynthesis: (1) resistance to photosensitized killing; (2) selection by tritium suicide; and (3) enzyme-targeted screening colony autoradiography. The specific used isolate animal that are deficient plasmalogens; variations of these methods have been many other lipid metabolism. Colony autoradiography can also be conjunction with techniques, because, quite often, only 1% the survivors a possess desired genotype. For instance, cells surviving suicide using [9,10- 3 H]hexadecanol screened, autoradiography, identify those which less able take up fatty alcohol. Other examples selected population reported. structure biosynthesis plasmalogens is described chapter. most mutant isolations—two lines—CHOK1 RAW 264.7 used. primary consideration both lines contain plasmenylethanolamine. Once isolates obtained, first thing must done determine if strains