Development of a Multifunctional Nanobiointerface Based on Self-Assembled Fusion-Protein rSbpA/ZZ for Blood Cell Enrichment and Phenotyping.

摘要: We present a multifunctional nanobiointerface for blood cell capture and phenotyping applications that features both excellent antifouling properties high antibody activity. Multifunctionality is accomplished by modifying polymeric materials using self-assembled S-layer fusion-protein rSbpA/ZZ to immobilize density antibodies at the two protein A binding sites of nanolattice structure. Controlled orientation alignment reduced consumption 100-fold increased efficiency 4-fold over standard methodologies. Cell analysis in complex samples was made possible remarkable rSbpA domain, while same time reducing unspecific forgoing tedious blocking procedures. An automated microfluidic situ platform isolation primary peripheral mononuclear cells developed as practical application. Results obtained our analysis...