ANNEXIN V BINDING AND MEROCYANINE STAINING FAIL TO DETECT HUMAN SPERM CAPACITATION.

摘要: ABSTRACT: The signaling pathways that characterize the process of capacitation human spermatozoa are still largely unknown. Modifications in lipid architecture sperm plasma membrane have been described from different species, including translocation phosphatidylserine (PS) inner to outer leaflet and increased phospholipid disorder membrane. In spermatozoa, however, results PS exposure controversial. present study, we used flow cytometry investigate both by Annexin V (Ann V) binding merocyanine 540 (M540) staining, swimup—selected live after incubation conditions leading capacitation. Our indicate neither probe is able detect capacitation-related modifications. Investigation nature M540-positive cells was then carried out. We found M540 stains elements devoid nuclei seminal plasma. Live PS-exposing were mainly represented damaged as revealed occurrence a negative correlation between normal morphology motility unselected samples. same also positive for M540. These demonstrate Ann samples detects with early degeneration well dead cells, which agreement findings obtained somatic two probes recognize due apoptotic process.