Urinary profiling of cis-diol-containing metabolites in rats with bisphenol A exposure by liquid chromatography-mass spectrometry and isotope labeling.
作者： Shangfu Li , Yibao Jin , Jue Wang , Zhi Tang , Shunqing Xu
DOI: 10.1039/C5AN02195B
关键词: Metabolism 、 Metabolite 、 Bisphenol A 、 Chromatography 、 Deuterated acetone 、 Urine 、 Mass spectrometry 、 Environmental chemistry 、 Acetone 、 Chemistry 、 Liquid chromatography–mass spectrometry
摘要: Exposure to bisphenol A (BPA), an environmental contaminant, has been linked metabolic disorders. However, there are no reports describing the effects of BPA on profiling cis-diol metabolites. It is challenge detect these metabolites in biological samples because their low abundance, high polarity and serious matrix interference. In this study, a chemical isotope-labeling method was applied solve problems. Acetone deuterated acetone (acetone-d6) were used as tags label rat urine samples, respectively. The light heavy labeling products recognized using ShiftedIonsFinder software. selected metabolite signals build data set. set evaluate changes urinary cis-diol-containing rats with exposure. results showed that chromatographic separation mass spectrometry detection improved after labeling. Using method, easily from samples. By comparing different dose administration rats, influence exposure investigated. analytes showing noticeable differences identified. found high-dose had strong compound metabolism. influences mostly related metabolism galactose nucleoside its analogues. disturbance by reported for first time, best our knowledge. This may have some implications exploring toxic
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