NAD glycohydrolase from the small intestine of the rat; purification, properties and possible role of the enzyme.

摘要: Abstract 1. In the investigation of NAD glycohydrolase (EC 3.2.2.5) in small intestine rat, properties this enzyme solubilized by lipase (glycerol-ester hydrolase, EC 3.1.1.3) and purified 250-fold were found to be similar essentials its other tissues. The Km values for NADP 2.7·10−5 3.8·10−6 M, respectively. 2. An over 70% inhibition hydrolysis was observed presence less than 1·10−6 M when used at a concentration 6.25·10−6 as substrate. contrast effect on cleavage, slight observed. 3. permeability pyridine nucleotide studied. No penetration [14C]NAD prior degradation [14C]nicotinamide This result would indicate that may contribute absorption moiety NAD, which significant diet.