Multidrug and Toxic Compound Extrusion-Type Transporters Implicated in Vacuolar Sequestration of Nicotine in Tobacco Roots
作者： Tsubasa Shoji , Koji Inai , Yoshiaki Yazaki , Yasutaka Sato , Hisabumi Takase
关键词: Structural gene 、 Nicotiana tabacum 、 Methyl jasmonate 、 Vacuole 、 Biology 、 Cytosol 、 Biochemistry 、 Saccharomyces cerevisiae 、 Nicotine 、 Jasmonate
摘要: Nicotine is a major alkaloid accumulating in the vacuole of tobacco (Nicotiana tabacum), but transporters involved vacuolar sequestration are not known. We here report that genes (NtMATE1 and NtMATE2) encoding multidrug toxic compound extrusion (MATE) family coordinately regulated with structural for nicotine biosynthesis root, respect to spatial expression patterns, regulation by NIC regulatory loci, induction methyl jasmonate. Subcellular fractionation, immunogold electron microscopy, green fluorescent protein fusion all suggested these localized membrane. Reduced rendered plants more sensitive application nicotine. In contrast, overexpression NtMATE1 cultured cells induced strong acidification cytoplasm after jasmonate elicitation or addition under nonelicited conditions. Expression yeast (Saccharomyces cerevisiae) compromised accumulation exogenously supplied into cells. The results imply MATE-type proteins transport alkaloids from cytosol exchange protons alkaloid-synthesizing root
plantphysiol.org
elsevier.com
cabdirect.org
jstor.org 参考文章(64)
Tsubasa Shoji, Robert Winz, Tadayuki Iwase, Keiji Nakajima, Yasuyuki Yamada, Takashi Hashimoto, Expression patterns of two tobacco isoflavone reductase-like genes and their possible roles in secondary metabolism in tobacco Plant Molecular Biology. ,vol. 50, pp. 427- 440 ,(2002) , 10.1023/A:1019867732278
T. C. Tso, Physiology and biochemistry of tobacco plants ,(1972)
Toshiyuki Nagata, Yasuyuki Nemoto, Seiichiro Hasezawa, Tobacco BY-2 Cell Line as the “HeLa” Cell in the Cell Biology of Higher Plants International Review of Cytology-a Survey of Cell Biology. ,vol. 132, pp. 1- 30 ,(1992) , 10.1016/S0074-7696(08)62452-3
Anja Paschold, Rayko Halitschke, Ian T. Baldwin, Co(i)-ordinating defenses: NaCOI1 mediates herbivore- induced resistance in Nicotiana attenuata and reveals the role of herbivore movement in avoiding defenses The Plant Journal. ,vol. 51, pp. 79- 91 ,(2007) , 10.1111/J.1365-313X.2007.03119.X
Changes in the activities of ornithine decarboxylase, putrescine N-methyltransferase and N-methylputrescine oxidase in tobacco roots in relation to nicotine biosynthesis Plant and Cell Physiology. ,vol. 14, pp. 103- 110 ,(1973) , 10.1093/OXFORDJOURNALS.PCP.A074831
Kathleen D. DeBoer, Jessica C. Lye, Campbell D. Aitken, Angela K.-K. Su, John D. Hamill, The A622 gene in Nicotiana glauca (tree tobacco): evidence for a functional role in pyridine alkaloid synthesis Plant Molecular Biology. ,vol. 69, pp. 299- 312 ,(2009) , 10.1007/S11103-008-9425-2
B. Deus-Neumann, M. H. Zenk, Accumulation of alkaloids in plant vacuoles does not involve an ion-trap mechanism Planta. ,vol. 167, pp. 44- 53 ,(1986) , 10.1007/BF00446367
Karen A. Cane, Melinda Mayer, Angela J. Lidgett, Anthony J. Michael, John D. Hamill, Molecular analysis of alkaloid metabolism in AABB v. aabb genotype Nicotiana tabacum in response to wounding of aerial tissues and methyl jasmonate treatment of cultured roots. Functional Plant Biology. ,vol. 32, pp. 305- 320 ,(2005) , 10.1071/FP04008
H Ito, Y Fukuda, K Murata, A Kimura, Transformation of intact yeast cells treated with alkali cations. Journal of Bacteriology. ,vol. 153, pp. 163- 168 ,(1983) , 10.1128/JB.153.1.163-168.1983
Masataka Kajikawa, Nobuhiro Hirai, Takashi Hashimoto, A PIP-family protein is required for biosynthesis of tobacco alkaloids. Plant Molecular Biology. ,vol. 69, pp. 287- 298 ,(2009) , 10.1007/S11103-008-9424-3