Actin-Modulating Protein Cofilin Is Involved in the Formation of Measles Virus Ribonucleoprotein Complex at the Perinuclear Region

摘要: UNLABELLED In measles virus (MV)-infected cells, the ribonucleoprotein (RNP) complex, comprised of viral genome and nucleocapsid (N) protein, phosphoprotein (P protein), large assembles at perinuclear region synthesizes RNAs. The cellular proteins involved in formation RNP complex are largely unknown. this report, we show that cofilin, an actin-modulating host interacts with MV N protein aids complex. Knockdown cofilin using short hairpin RNA reduces after infection complex-like structure plasmid-mediated expression P proteins. A lower level results reduction synthesis. Cofilin phosphorylation on serine residue position 3, enzymatically inactive form, is increased phosphorylated form preferentially included These indicate plays important role replication by increasing IMPORTANCE Many viruses induce within infected cells called which synthesis occurs. It include polymerase. binds to (MV) inactive, infection, associated Our findings determined will help us better understand mechanism formed virus-infected develop new antiviral drugs targeting