Increased Production of Interleukin-8 in Primary Human Monocytes and in Human Epithelial and Endothelial Cell Lines after Dengue Virus Challenge

摘要: The more severe form of dengue virus infection, hemorrhagic fever, is characterized by plasma leakage and derangements in hemostasis. As elevated interleukin-8 (IL-8) levels have been observed sera from patients with disease manifestations, a study was initiated to look at the effect infection vitro on proinflammatory cytokine secretion expression. A significant increase IL-8 culture supernatant primary human monocytes infected 2 (D2V) New Guinea C (NGC) found enzyme-linked immunosorbent assay. Additionally, reverse transcriptase PCR, mRNA also augmented. Among cytokines their mRNAs measured (IL-6, IL-1 beta, IL-8, tumor necrosis factor alpha), showed greatest change following D2V infection. Similarly, two cell lines, 293T (a epithelial line) ECV304 (an endothelial line), were permissive NGC responded increasing synthesis IL-8. Nuclear kappa B (NF-kappa B) nuclear IL-6 (NFIL-6) are mediators We studied transcriptional regulation lines that induction gene expression involved activation NF-kappa (P = 0.001) and, lesser extent, NFIL-6 cells only. next chromatin immunoprecipitation procedure vivo acetylation core histones bound promoter after produced may be or other associated hyperacetylation addition transcription B. hypothesize overall this play role pathogenesis seen fever shock syndrome.