Microbiological monitoring of sheep carcass contamination in three Swiss abattoirs.

摘要: At three Swiss abattoirs, 580 sheep carcasses were examined at 10 sites by the wet-dry double-swab technique. The aim of this study was to obtain data on microbiological contamination abattoirs and develop a procedure for monitoring slaughter hygiene. Median aerobic plate counts (APCs) (log CFU/cm 2 ) ranged from 2.5 3.8, with brisket neck showing most extensive contamination. Enterobacteriaceae detected 68.1% in 15.2% samples. proportion positive results 2.6% (for hind leg flank abattoir C) 42.2% perineal area A). percentage samples testing stx genes polymerase chain reaction 36.6%. A significant relationship between APC detection Shiga toxin-producing Escherichia coli (STEC) found B (depending sampling site), whereas STEC confirmed only (P < 0.05). In 57.1% 56 isolated non-0157 strains, stx2 detected, them stx2d positive. Additional virulence factors 50% 8.9% these strains being eae positive, EHEC-hlyA 3.6% astA For determination carcass contamination, monthly examination neck, brisket, areas is recommended. This valuable tool verification hygiene according hazard analysis critical control point principles.