Combining functional imaging and interstitial pressure measurements to evaluate two anti-angiogenic treatments

摘要: Background: Interstitial hypertension is responsible for poor capillary blood flow and hampered drug delivery. The efficacy of combined sorafenib/bevacizumab treatment given according to different administration schedules has been evaluated by measuring both interstitial pressure (IP) quantitative dynamic contrast-enhanced ultrasonography (DCE-US) parameters in melanoma-bearing mice. Materiel Methods: Sixty mice were xenografted with B16F10 melanoma. Animals received a daily over 4 days (D0 D3) either sorafenib at 30 mg/kg, bevacizumab 2.5 mg/kg alone, or treatments. Perfusion determined using an Aplio® sonograph (Toshiba) SonoVue® contrast agent (Bracco) compared IP measurements fiberoptic probes (Samba®) D0, D2, D4, D8. Results: mean baseline values ranged between 6.55 31.29 mmHg all the groups. A transient decrease occurred D2 treated groups, especially concomitant group which exhibited significant reduction D0. peak intensity area under curve was observed D4 molecules yielded maximal inhibition tumor volume number vessels. No correlation found perfusion parameters, indicating complex relationships vascularization. gradients center periphery but these two regions significantly correlated (R = 0.93). Conclusion: results suggest that variations could be predictive vascular changes one single measurement sufficient fully characterize whole tumor.