Lysosomal hyaluronidase from rat liver. I. Preparation.
作者： Nathan N. Aronson , Eugene A. Davidson
DOI: 10.1016/S0021-9258(18)96291-5
关键词: Polyacrylamide gel electrophoresis 、 Hydroxylapatite 、 Sedimentation equilibrium 、 Chromatography 、 Hyaluronidase 、 Electrophoresis 、 Ammonium sulfate precipitation 、 Chemistry 、 Ultracentrifuge 、 Sephadex 、 Biochemistry
摘要: Hyaluronidase isolated from the lysosomes of rat liver (1) was purified 1300-fold by ammonium sulfate precipitation, chromatography on hydroxylapatite, and Sephadex G-100. The most fraction gave two peaks sedimentation in ultracentrifuge, faster being yellow possessing no enzymic activity. slower peak showed a single band acrylamide gel electrophoresis. molecular weight enzyme calculated to be 89,000 based equilibrium data.
sci-hub.st 参考文章(34)
Edward D. Korn, The isolation of heparin from mouse mast cell tumor. Journal of Biological Chemistry. ,vol. 234, pp. 1325- 1329 ,(1959) , 10.1016/S0021-9258(18)70004-5
Bernard Weissmann, THE TRANSGLYCOSYLATIVE ACTION OF TESTICULAR HYALURONIDASE Journal of Biological Chemistry. ,vol. 216, pp. 783- 794 ,(1955) , 10.1016/S0021-9258(19)81432-1
J. E. Scott, Aliphatic Ammonium Salts in the Assay of Acidic Polysaccharides from Tissues Methods of Biochemical Analysis. ,vol. 8, pp. 145- 197 ,(2006) , 10.1002/9780470110249.CH4
C. de Duve, B. C. Pressman, R. Gianetto, R. Wattiaux, F. Appelmans, Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue* Biochemical Journal. ,vol. 60, pp. 604- 617 ,(1955) , 10.1042/BJ0600604
Norton Nelson, A PHOTOMETRIC ADAPTATION OF THE SOMOGYI METHOD FOR THE DETERMINATION OF GLUCOSE Journal of Biological Chemistry. ,vol. 153, pp. 375- 380 ,(1944) , 10.1016/S0021-9258(18)71980-7
Monroe E. Freeman, Pearl. Anderson, Marian. Oberg, Albert. Dorfman, Preparation of purified hyaluronidase from bovine testis. Journal of Biological Chemistry. ,vol. 180, pp. 655- 662 ,(1949) , 10.1016/S0021-9258(18)56684-9
José L Reissig, Jack L Strominger, Luis Federico Leloir, A MODIFIED COLORIMETRIC METHOD FOR THE ESTIMATION OF N-ACETYLAMINO SUGARS Journal of Biological Chemistry. ,vol. 217, pp. 959- 966 ,(1955) , 10.1016/S0021-9258(18)65959-9
Nathan N. Aronson, Eugene A. Davidson, Lysosomal Hyaluronidase from Rat Liver II. PROPERTIES Journal of Biological Chemistry. ,vol. 242, pp. 441- 444 ,(1967) , 10.1016/S0021-9258(18)96292-7
I. Danishefsky, A. Bella, The Sulfated Mucopolysaccharides from Human Umbilical Cord Journal of Biological Chemistry. ,vol. 241, pp. 143- 146 ,(1966) , 10.1016/S0021-9258(18)96969-3
N. Katsura, E.A. Davidson, Studies on porcine costal catillage protein-polysaccharide complex Biochimica et Biophysica Acta (BBA) - General Subjects. ,vol. 121, pp. 120- 127 ,(1966) , 10.1016/0304-4165(66)90354-0