In vitro response to alkaline phosphatase coatings immobilized onto titanium implants using electrospray deposition or polydopamine-assisted deposition.
作者： Arnold W. G. Nijhuis , Jeroen J.J.P. van den Beucken , John A. Jansen , Sander C. G. Leeuwenburgh
DOI: 10.1002/JBM.A.34776
关键词: Titanium 、 Biochemistry 、 Biomolecule 、 Mineralization (soil science) 、 Materials science 、 Immobilized enzyme 、 Implant 、 Electrospray 、 Alkaline phosphatase 、 Chemical engineering 、 Surface modification
摘要: Immobilization of biomolecules onto implant surfaces is one the most straightforward strategies to control interaction between an and its biological environment. Recently, it was shown that enzyme alkaline phosphatase (ALP) could be efficiently immobilized titanium implants in a single step using polydopamine. We hypothesized such polydopamine-ALP coatings can enhance early attachment cells increase mineralization. Therefore, current study aimed at immobilization ALP by means either one- or two-step polydopamine-assisted electrospray deposition, comparative characterization these experimental substrates subsequent cell behavioral analysis primary osteoblast-like cells. Uncoated ALP-free polydopamine served as controls. Despite significant surface activity lower water contact for angles ALP-containing modifications, only marginal effects on behavior (i.e., spreading) osteogenic differentiation proliferation, mineralization) were observed comparison uncoated titanium. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1102-1109, 2014.
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