Tumor suppressor p16INK4A: determination of solution structure and analyses of its interaction with cyclin-dependent kinase 4.
作者： In-Ja L. Byeon , Junan Li , Karen Ericson , Thomas L. Selby , Anton Tevelev
DOI: 10.1016/S1097-2765(00)80042-8
关键词: Suppressor 、 Cyclin-dependent kinase 4 、 Mutant 、 N-terminus 、 Protein tertiary structure 、 Mutational analysis 、 Molecular biology 、 Biochemistry 、 Docking (molecular) 、 Biology 、 Solution structure
摘要: The solution structure of the tumor suppressor p16INK4A has been determined by NMR, and important recognition regions both cdk4 have identified. tertiary contains four helix-turn-helix motifs linked three loops. Twelve tumorigenic mutants constructed analyzed for their activity, new designed rationally. A fragment 58 residues at N terminus binding importance this region was further verified mutational analysis cdk4. These results docking experiments used to assess possible modes between
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