Oligosaccharide structures of mucins secreted by the human colonic cancer cell line CL.16E.
作者： C Capon , C.L. Laboisse , J.M. Wieruszeski , J.J. Maoret , C Augeron
DOI: 10.1016/S0021-9258(18)41768-1
关键词: Glycoprotein 、 Fucose 、 Chemistry 、 Biochemistry 、 Nuclear magnetic resonance spectroscopy 、 Stereochemistry 、 Size-exclusion chromatography 、 Sialic acid 、 Mucin 、 Protein primary structure 、 Oligosaccharide
摘要: Cl.16E, a stably differentiated clonal derivative of the human colonic cancer cell line HT29, was used to investigate structure oligosaccharide chains mucins in cancer. Secretory were purified by equilibrium density gradient centrifugation CsCl. Oligosaccharide side isolated after beta-elimination. Compositional analysis oligosaccharide-alditols performed purification gel filtration on Bio-gel P-6 column showed 1) that GalNAc residues located exclusively at reducing ends chains, and 2) fucose absent from preparation. Oligosaccharide-alditols separated high performance liquid chromatography (HPLC) quaternary amine packings into minor neutral fraction representing about 6.5% weight released oligosaccharides four acidic fractions. Two fractions, namely FI FII encompassing mono- disialylated structures, respectively, containing 78% total alditols, HPLC. Structural determinations carried out using methylation analysis, 1H NMR spectroscopy, fast atom bombardment-mass spectrometry. Twelve structures determined which ranged size 3 8 residues. These based core types 1, 2, 4. Elongation terminated addition sialic acid alpha 2-3 linkage Gal beta 1-3R 1-4R The predominant hexasaccharide (fraction FII-4). This contrasts with normal whose previously found be carry acids (2-6) 1-3R, 1-4R, alpha-R (Podolsky, D.K. (1985) J. Biol. Chem. 260, 8262-8271; Podolsky, 15510-15515). Collectively our findings suggest Cl.16E colon cells are able synthesize mucin gastric type elongation is truncated premature sialylation.
sci-hub.st 参考文章(47)
A Kurosaka, H Nakajima, I Funakoshi, M Matsuyama, T Nagayo, I Yamashina, Structures of the major oligosaccharides from a human rectal adenocarcinoma glycoprotein. Journal of Biological Chemistry. ,vol. 258, pp. 11594- 11598 ,(1983) , 10.1016/S0021-9258(17)44269-4
H Van Halbeek, L Dorland, J.F.G Vliegenthart, A.-M Fiat, P Jolles, A 360-MHz 1H-NMR study of three oligosaccharides isolated from cow κ-casein Biochimica et Biophysica Acta. ,vol. 623, pp. 295- 300 ,(1980) , 10.1016/0005-2795(80)90257-3
D K Podolsky, Oligosaccharide structures of human colonic mucin. Journal of Biological Chemistry. ,vol. 260, pp. 8262- 8271 ,(1985) , 10.1016/S0021-9258(17)39465-6
R. N. Taub, A. Kanani, K. L. Matta, D. R. Sutherland, A. Hindenburg, M. A. Baker, I. Brockhausen, E. Fibach, W. Kuhns, D. H. Van Den Eijnden, Human Leukemic Myeloblasts and Myeloblastoid Cells Contain the Enzyme Cytidine 5′-Monophosphate-N-acetylneuraminic Acid:Galβ1-3GalNacα(2–3)-sialyltransferase Cancer Research. ,vol. 50, pp. 5003- 5007 ,(1990)
A Kurosaka, H Kitagawa, S Fukui, Y Numata, H Nakada, I Funakoshi, T Kawasaki, T Ogawa, H Iijima, I Yamashina, A monoclonal antibody that recognizes a cluster of a disaccharide, NeuAc alpha(2----6)GalNAc, in mucin-type glycoproteins. Journal of Biological Chemistry. ,vol. 263, pp. 8724- 8726 ,(1988) , 10.1016/S0021-9258(18)68365-6
D K Podolsky, Oligosaccharide structures of isolated human colonic mucin species. Journal of Biological Chemistry. ,vol. 260, pp. 15510- 15515 ,(1985) , 10.1016/S0021-9258(17)36284-1
B L Slomiany, E Zdebska, A Slomiany, Structural characterization of neutral oligosaccharides of human H+Leb+ gastric mucin. Journal of Biological Chemistry. ,vol. 259, pp. 2863- 2869 ,(1984) , 10.1016/S0021-9258(17)43227-3
V Piller, F Piller, M Fukuda, Biosynthesis of truncated O-glycans in the T cell line Jurkat. Localization of O-glycan initiation. Journal of Biological Chemistry. ,vol. 265, pp. 9264- 9271 ,(1990) , 10.1016/S0021-9258(19)38842-8
A Slomiany, E Zdebska, B L Slomiany, Structures of the neutral oligosaccharides isolated from A-active human gastric mucin. Journal of Biological Chemistry. ,vol. 259, pp. 14743- 14749 ,(1984) , 10.1016/S0021-9258(17)42665-2
Alain Barbat, Elisabeth Dussaulx, Alain Zweibaum, Thécla Lesuffleur, Growth adaptation to methotrexate of HT-29 human colon carcinoma cells is associated with their ability to differentiate into columnar absorptive and mucus-secreting cells. Cancer Research. ,vol. 50, pp. 6334- 6343 ,(1990)