Upregulation of transcripts for metabolism in diverse environments is a shared response associated with survival and adaptation of Klebsiella pneumoniae in response to temperature extremes

摘要: Klebsiella pneumoniae being ubiquitous in nature encounters wide differences environmental condition. The organism’s abundance natural water reservoirs exposed to temperature variation forms the basis of its persistence and spread soil other farm produce. In order investigate effect changes on survival adaptation bacteria, transcriptional response K. subjected low (20 °C) high (50 °C) shock were executed using Applied Biosystems SOLiD platform. Approximately, 33 34 % protein coding genes expressed 20 50 °C, respectively, displayed significant up- or downregulation (p < 0.01). Most significantly transcripts mapped metabolism, membrane transport, cell motility downregulated at except for folding, sorting, degradation, suggesting that heat stress causes general gene expression together with induction proteins. While 20 °C, carbohydrate, lipid, amino acid metabolism highly upregulated. Hypothetical proteins as well canonical cold proteins, viz. grpE, clpX, recA, deaD upregulated commonly 50 °C. Significant upregulation encoding ribosomal 50 °C possibly suggest their role cells under low- high-temperature stress.