Disruption of genes encoding pyruvate dehydrogenase kinases leads to retarded growth on acetate and ethanol in Saccharomyces cerevisiae.
作者： H. Yde Steensma , Lubomir Tomaska , Peter Reuven , Jozef Nosek , Raymond Brandt
DOI: 10.1002/YEA.1543
关键词: Oxoglutarate dehydrogenase complex 、 Dihydrolipoyl transacetylase 、 Molecular biology 、 Biochemistry 、 Pyruvate decarboxylation 、 Pyruvate dehydrogenase kinase 、 Citric acid cycle 、 Branched-chain alpha-keto acid dehydrogenase complex 、 Pyruvate dehydrogenase complex 、 Biology 、 Pyruvate dehydrogenase phosphatase
摘要: Two open reading frames, YIL042c (PKP1) and YGL059w, with 25% sequence similarity to human pyruvate dehydrogenase kinases, were shown have protein kinase activity. Using GFP fusions, it was demonstrated that the proteins localize in discrete submitochondrial regions. Strains a null mutation these loci grew poorly on acetate ethanol as carbon sources. Doubling times increased from ca. 4 h wild-type > 6 for mutants. Growth rates of mutants could be restored levels by simultaneous disruption PDA1 gene, encoding E1α subunit complex. This observation activities measured mutant strains grown glucose or suggest slow growth phenotype C2 sources is caused futile cycle which phosphoenolpyruvate converted back acetyl coenzyme A. Copyright © 2007 John Wiley & Sons, Ltd.
sci-hub.se 参考文章(31)
Jitao Zou, Qungang Qi, Vesna Katavic, Elizabeth-F. Marillia, David C. Taylor, Effects of antisense repression of an Arabidopsis thaliana pyruvate dehydrogenase kinase cDNA on plant development. Plant Molecular Biology. ,vol. 41, pp. 837- 849 ,(1999) , 10.1023/A:1006393726018
George-B. KRESZE, Hedwig RONFT, Pyruvate Dehydrogenase Complex from Baker's Yeast FEBS Journal. ,vol. 119, pp. 573- 579 ,(1981) , 10.1111/J.1432-1033.1981.TB05646.X
Mohammed Rahmatullah, Thomas E Roche, None, Component requirements for NADH inhibition and spermine stimulation of pyruvate dehydrogenaseb phosphatase activity. Journal of Biological Chemistry. ,vol. 263, pp. 8106- 8110 ,(1988) , 10.1016/S0021-9258(18)68448-0
Ulrich Güldener, Susanne Heck, Thomas Fiedler, Jens Beinhauer, Johannes H Hegemann, A New Efficient Gene Disruption Cassette for Repeated Use in Budding Yeast Nucleic Acids Research. ,vol. 24, pp. 2519- 2524 ,(1996) , 10.1093/NAR/24.13.2519
R.Daniel Gietz, Sugino Akio, New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene. ,vol. 74, pp. 527- 534 ,(1988) , 10.1016/0378-1119(88)90185-0
Brian H. Robinson, Cell culture studies on patients with mitochondrial diseases: molecular defects in pyruvate dehydrogenase. Journal of Bioenergetics and Biomembranes. ,vol. 20, pp. 313- 323 ,(1988) , 10.1007/BF00769635
A.Gordon James, Ritchard M. Cook, Shauna M. West, J.Gordon Lindsay, The pyruvate dehydrogenase complex of Saccharomyces cerevisiae is regulated by phosphorylation FEBS Letters. ,vol. 373, pp. 111- 114 ,(1995) , 10.1016/0014-5793(95)01020-F
J. Dietvorst, L. Blieck, R. Brandt, P. Van Dijck, H. Y. Steensma, Attachment of MAL32-encoded maltase on the outside of yeast cells improves maltotriose utilization. Yeast. ,vol. 24, pp. 27- 38 ,(2007) , 10.1002/YEA.1436
George-B. KRESZE, Hedwig RONFT, Pyruvate dehydrogenase complex from baker's yeast. 2. Molecular structure, dissociation, and implications for the origin of mitochondria. FEBS Journal. ,vol. 119, pp. 581- 587 ,(1981) , 10.1111/J.1432-1033.1981.TB05647.X
Edith Bogengruber, Peter Briza, Edith Doppler, Herbert Wimmer, Lore Koller, Franco Fasiolo, Bruno Senger, Johannes H Hegemann, Michael Breitenbach, Functional analysis in yeast of the Brix protein superfamily involved in the biogenesis of ribosomes Fems Yeast Research. ,vol. 3, pp. 35- 43 ,(2003) , 10.1111/J.1567-1364.2003.TB00136.X