Fusarium graminearum pyruvate dehydrogenase kinase 1 (FgPDK1) Is Critical for Conidiation, Mycelium Growth, and Pathogenicity.
作者： Tao Gao , Jian Chen , Zhiqi Shi
DOI: 10.1371/JOURNAL.PONE.0158077
关键词:
摘要: Pyruvate dehydrogenase kinase (PDK) is an important mitochondrial enzyme that blocks the production of acetyl-CoA by selectively inhibiting activity pyruvate (PDH) through phosphorylation. PDK effectively therapeutic target in cancer cells, but physiological roles phytopathogens are largely unknown. To address these gaps, a gene (FgPDK1) was isolated from Fusarium graminearum economically pathogen infecting cereals. The deletion FgPDK1 F. resulted increase PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure perithecia and conidia production, pigment formation. ΔFgPDK1 mutants showed enhanced sensitivity to osmotic stress cell membrane-damaging agent. Physiological detection indicated reactive oxygen species (ROS) accumulation plasma membrane damage (indicated PI staining, lipid peroxidation, electrolyte leakage) occurred mutants. also prohibited deoxynivalenol (DON) pathogenicity graminearum, which may decrease expression Tri6. Taken together, this study firstly identified vital development phytopathogen provide potentially novel clue for target-directed agricultural fungicides.
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