Molecular cloning and functional analysis of the phosphomannomutase (PMM) gene from Dendrobium officinale and evidence for the involvement of an abiotic stress response during germination.
作者： Chunmei He , Songjun Zeng , Jaime A. Teixeira da Silva , Zhenming Yu , Jianwen Tan
DOI: 10.1007/S00709-016-1044-1
关键词: Ascorbic acid 、 Abscisic acid 、 Biology 、 Abiotic stress 、 Dendrobium 、 Complementary DNA 、 Biochemistry 、 Gene expression 、 Arabidopsis thaliana 、 Phosphomannomutase
摘要: Phosphomannomutase (PMM, EC 5.4.2.8) catalyzes the interconversion of mannose-6-phosphate to mannose-1-phosphate, precursor for synthesis GDP-mannose. In this study, complementary DNA (cDNA) (PMM) gene was initially cloned from Dendrobium officinale by RACE method. Transient transform result showed that DoPMM protein localized in cytoplasm. The highly expressed stems D. both vegetative and reproductive developmental stages. putative promoter TAIL-PCR used searched cis-elements. Stress-related cis-elements like ABRE, TCA-element, MBS were found regions. up-regulated after treatment with abscisic acid, salicylic cold, polyethylene glycol, NaCl. total ascorbic acid (AsA) polysaccharide content all 35S::DoPMM Arabidopsis thaliana transgenic lines #1, #2, #5 a 40, 39, 31% increase AsA 77, 22, 39% polysaccharides, respectively more than wild-type (WT) levels. All three exhibited higher germination percentage WT plants when seeded on half-strength MS medium supplemented 150 mM NaCl or 300 mM mannitol. These results provide genetic evidence involvement PMM genes biosynthesis polysaccharides mediation abiotic stress tolerance during seed A. thaliana.
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