Genome-Wide Identification and Analysis of the APETALA2 (AP2) Transcription Factor in Dendrobium officinale.
作者： Chunmei He , Zhenming Yu , Jaime A. Teixeira da Silva , Can Si , Mingze Zhang
DOI: 10.3390/IJMS22105221
关键词:
摘要: The APETALA2 (AP2) transcription factors (TFs) play crucial roles in regulating development plants. However, a comprehensive analysis of the AP2 family members valuable Chinese herbal orchid, Dendrobium officinale, or other orchids, is limited. In this study, 14 DoAP2 TFs that were identified from D. officinale genome and named DoAP2-1 to DoAP2-14 divided into three clades: euAP2, euANT, basalANT. promoters all genes contained cis-regulatory elements related plant also responsive hormones stress. qRT-PCR showed abundant expression DoAP2-2, DoAP2-5, DoAP2-7, DoAP2-8 DoAP2-12 protocorm-like bodies (PLBs), while DoAP2-3, DoAP2-4, DoAP2-6, DoAP2-9, DoAP2-10 DoAP2-11 was strong plantlets. addition, some down-regulated during flower development. These results suggest may regeneration officinale. Four (DoAP2-1 DoAP2-2 DoAP2-6 basal ANT) selected for further analyses. transcriptional activation DoAP2-1, proteins, which localized nucleus Arabidopsis thaliana mesophyll protoplasts, analyzed by dual-luciferase reporter gene system Nicotiana benthamiana leaves. Our data pBD-DoAP2-1, pBD-DoAP2-2, pBD-DoAP2-6 pBD-DoAP2-11 significantly repressed LUC compared with negative control (pBD), suggesting these proteins act as repressors cells. findings on shed light function orchid species.
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