Impact of bio-augmentation with Sphingomonas sp. strain TTNP3 in membrane bioreactors degrading nonylphenol

作者: Magdalena Cirja , Gregor Hommes , Pavel Ivashechkin , Jürgen Prell , Andreas Schäffer

DOI: 10.1007/S00253-009-2050-4

关键词: SphingomonasMixed liquor suspended solidsChemistryEffluentActivated sludgeChromatographyBioaugmentationNonylphenolMembrane reactorBioreactor

摘要: This study evaluates the potential of bio-augmentation to improve degradation recalcitrant nonylphenol during wastewater treatment in membrane bioreactors (MBR). One MBR containing activated sludge was bio-augmented using multistep inoculation with freeze dried Sphingomonas sp. strain TTNP3, whereas a second control reactor contained solely. The 14C-labeled-nonylphenol isomer (4-[1-ethyl-1,3-dimethylpentyl]phenol) applied as single pulse. Bio-augmentation resulted an immediate increase dissolved radioactivity effluent comparison (13% and 2% initially after 1 day, respectively). After 5 days operation, retentate only 7% initial contrast 50% reactor. associated mixed liquor suspended solids, i.e., suspension biomass other solids on side membrane, mainly found non-extractable residues that were increasingly formed prolonged especially for MBR. HPLC-LSC GC-MSn analyses revealed produced more polar hydroquinone main intermediate, complex mixture apolar compounds shortened oxidized alkyl chains. Thus, apparent differences behavior between reactors due catabolism conferred by TTNP3.

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