A convenient method for multicolour labelling of proteins with BODIPY fluorophores via tyrosine residues
作者： Miffy. H. Y. Cheng , Huguette Savoie , Francesca Bryden , Ross. W. Boyle
DOI: 10.1039/C7PP00091J
关键词: Fluorescence 、 Confocal microscopy 、 Labelling 、 BODIPY 、 Tyrosine 、 Biochemistry 、 HeLa 、 Chemistry 、 Globular protein 、 Fluorescence-lifetime imaging microscopy
摘要: Fluorescence is an essential imaging modality for labelling and visualising cells sub-cellular structures. Multicolour especially challenging due to differences in physicochemical photophysical behaviour of structurally unrelated fluorophores the heterogeneous environments found compartments. Herein, we report conjugation three azide-bearing BODIPYs with similar core structures but widely different emission wavelengths (green, red NIR) tyrosine residues a model globular protein (BSA) via common linking methodology. The resulting BODIPY–BSA conjugates have demonstrated multi-wavelength fluorescence biological applications. was performed HeLa through live cell confocal microscopy imaging, good intracellular location visualisation observed.
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