Evaluation of a radioimmunoassay for the measurement of amphibian parathyroid hormone.
作者： Lawrence W. Wittle , Hannalore Rupprecht , Heather Ruppel , Kurt Caugh
DOI: 10.1016/0016-6480(92)90158-G
关键词: Quantitative analysis (chemistry) 、 Coefficient of variation 、 Biology 、 Salientia 、 Parathyroidectomy 、 Parathyroid hormone 、 Endocrinology 、 Amphibian 、 Radioimmunoassay 、 Internal medicine 、 Calcium
摘要: A commercial mid-region to carboxyl-region parathyroid hormone (PTH) radioimmunoassay (RIA) for measurement of PTH concentration in serum humans was evaluated its ability detect amphibian PTH. The recovery known amounts human added aliquots pooled frog demonstrated that does not interfere with the RIA system. mean intraassay coefficient variation 0.11. During spring and summer removal glands from frogs resulted significant decreases calcium levels which revealed were associated levels. In winter showed histological changes inactivity. Also, parathyroidectomy did affect concentrations, experimental control animals below or near level detection.
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