Effects of bone morphogenic protein-2 loaded on the 3D-printed MesoCS scaffolds.

摘要: Background/Purpose The mesoporous calcium silicate (MesoCS) 3D-printed scaffold show excellent bioactivity and can enhance the bone-like apatite formation. purpose of this study aims to consider effects different loading methods on novel grafting materials which composed bone morphogenetic protein-2 (BMP-2) loaded MesoCS by employing 3D-printing technique. Methods were fabricated fused deposition modeling. In study, there are two BMP-2: (1) pre-loading (PL) method mixing BMP-2 as a raw material for 3D-printer, (2) direct-loading (DL) soaking in solution. characteristics examined transmission electron microscopy (TEM), X-ray diffraction (XRD) scanning (SEM). Their physical properties, biocompatibility, osteogenic-related ability also evaluated. Results 3D MesoCS/PCL scaffolds showed biocompatibility properties. After simulated body fluid, layer PL DL groups could be formed. addition, group released fifty percent more than at end first day sustained release profile after 2 weeks. Conclusion porous successfully via printing system tested in vitro found good cellular activity cell behavior although was not favorable clinical application relation with preservation BMP-2. With regards growth factor methods, demonstrated that into prior will result drug pattern compared traditional directly immersed