A hammerhead ribozyme suppresses expression of hepatocyte growth factor/scatter factor receptor c-MET and reduces migration and invasiveness of breast cancer cells

摘要: PURPOSE: Hepatocyte growth factor/scatter factor (HGF/SF), via its receptor c-MET, has been implicated to play a pivotal role in breast cancer development and progression. This study examined transgene-consisting of combination U1snRNA, hammerhead ribozyme, antisense, designed inhibit c-met expression-and impact on the migration vitro invasion cells. EXPERIMENTAL DESIGN: A ribozyme targeting human c-MET was cloned into modified pZeoU1EcoSpe vector transfected cells MDA MB 231 MCF-7 by electroporation. Expression MET mRNA protein determined. Migration invasiveness were also analyzed. RESULTS: Breast with ribozyme-containing plasmids. Stable transfectants manifested an almost complete loss protein, as shown reverse transcription-PCR, Northern blotting, Western respectively, whereas wild-type plasmid had no effects. Met-ribozyme exhibited reduced through extracellular matrix (Matrigel), compared empty plasmid. CONCLUSIONS: These data show that way encoding antisense is effective approach reducing cells.