Deletion of the cell adhesion adaptor protein vinculin disturbs the localization of GFAP in Bergmann glial cells

摘要: Astrocytes operate in close spatial relationship to other cells including neurons. Structural interaction is controlled by adynamic interplay between actin-based cell motility and contact formation via cell–cell cell–extracellular matrix adhesions.A central player the control of adhesion cytoskeletal adaptor protein Vinculin. Incorporation Vinculin affectsmechanical properties turnover sites. To study vivo function astrocytes, a mouse linewith astrocyte specific inducible deletion vinculin was generated. Deletion decreased expression theglial acidic fibrillary (GFAP) Bergmann glial cerebellum. In addition, localization GFAP Bergmannglial endfeet disturbed, indicating role for controlling its localization. contrast, vimentinexpression, morphology, activation state polarity targeted as well extracellularmatrix laminin not compromised. Furthermore, stab wound lesions were performed cerebellar cortex. Inboth wildtype knockout mice upregulated lesioned area withno differences observed genotypes GFAP. These results propose selectiverequirement cellular events related vivo.Asin vitro data suggested major vinculinin connection affecting mechanical stability motility, our add note caution tothe extrapolation function.GLIA 2013;61:1067–1083Key words: astrocyte; vinculin; type knockout; GFAP; CreERT2