Vinculin facilitates cell invasion into three-dimensional collagen matrices.
作者： Claudia T Mierke , Philip Kollmannsberger , Daniel Paranhos Zitterbart , Gerold Diez , Thorsten M Koch
关键词: Cell adhesion 、 Motility 、 Biology 、 Cytoskeleton 、 Cell migration 、 Vinculin 、 Focal adhesion 、 Extracellular matrix 、 Cell biology 、 Actin
摘要: The cytoskeletal protein vinculin contributes to the mechanical link of contractile actomyosin cytoskeleton extracellular matrix (ECM) through integrin receptors. In addition, modulates dynamics cell adhesions and is associated with decreased motility on two-dimensional ECM substrates. effect invasion dense three-dimensional gels unknown. Here, we report how expression affects into collagen matrices. Cell was investigated in knockout expressing wild-type mouse embryonic fibroblasts. Vinculin cells were 2-fold more motile collagen-coated substrates compared cells, but 3-fold less invasive 2.4 mg/ml softer remodeled their dynamically, which consistent enhanced does not explain reduced invasiveness. Importantly, vinculin-expressing adhered strongly generated higher traction forces cells. Moreover, able migrate (5.8 mg/ml) matrices that impenetrable for These findings suggest facilitates up-regulation or transmission are needed overcome steric hindrance ECMs.
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