Mouse model of muscle crush injury of the legs
作者： Nadia D Fulkerson , Barbara St Pierre Schneider , Jennifer Nicholas , Georgina L Dobek
DOI:
关键词: Reduction (orthopedic surgery) 、 H&E stain 、 Pathology 、 Fibular fracture 、 Skeletal muscle 、 Regeneration (biology) 、 Medicine 、 Macrophage infiltration 、 Crush syndrome 、 Crush injury
摘要: Because crush injury to skeletal muscle is an important cause of morbidity in natural disaster and battlefield settings, a reproducible refined animal model needed. Both open closed small-animal models are available but limited by their need for surgical isolation the or adverse effect fibular fracture, respectively. In current study, we developed validated novel, noninvasive mouse lower-extremity injury. Despite nature our model, gross evidence damage was evident all mice verified microscopically through hematoxylin eosin staining. The elicited both neutrophil macrophage infiltration at 24 48 h after area percentage mean antigen F4/80-positive macrophages were higher than injury, CD68-positive differed significantly between injured uninjured muscle. addition, incidence fracture one third lower that reported alternative model. conclusion, method pelvic limb refinement previous because its decreased bone fractures reduction numbers.
参考文章(20)
Nicole M McBrier, Craig R Denegar, Thomas Neuberger, Neil A Sharkey, Andrew G Webb, Magnetic resonance imaging of acute injury in rats and the effects of buprenorphine on limb volume. Journal of The American Association for Laboratory Animal Science. ,vol. 48, pp. 147- 151 ,(2009)
James M. Zouris, G. Jay Walker, Judy Dye, Michael Galarneau, Wounding patterns for U.S. Marines and sailors during Operation Iraqi Freedom, major combat phase. Military Medicine. ,vol. 171, pp. 246- 252 ,(2006) , 10.7205/MILMED.171.3.246
T R Malek, T J Fleming, M L Fleming, Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. Journal of Immunology. ,vol. 151, pp. 2399- 2408 ,(1993)
Tobias Winkler, Philipp von Roth, Georg Matziolis, Maria R Schumann, Sebastian Hahn, Patrick Strube, Gisela Stoltenburg-Didinger, Carsten Perka, Georg N Duda, Stephan V Tohtz, Time course of skeletal muscle regeneration after severe trauma. Acta Orthopaedica. ,vol. 82, pp. 102- 111 ,(2011) , 10.3109/17453674.2010.539498
Ursula Malorny, Elmar Michels, Clemens Sorg, A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes. Cell and Tissue Research. ,vol. 243, pp. 421- 428 ,(1986) , 10.1007/BF00251059
Rosângela P. da SILVA, Siamon GORDON, Phagocytosis stimulates alternative glycosylation of macrosialin (mouse CD68), a macrophage-specific endosomal protein Biochemical Journal. ,vol. 338, pp. 687- 694 ,(1999) , 10.1042/BJ3380687
Uta Kerkweg, Daniel Schmitz, Herbert de Groot, Screening for the formation of reactive oxygen species and of NO in muscle tissue and remote organs upon mechanical trauma to the mouse hind limb. European Surgical Research. ,vol. 38, pp. 83- 89 ,(2006) , 10.1159/000092609
John B. Kurek, Sara Nouri, George Kannourakis, Mark Murphy, Lawrence Austin, Leukemia inhibitory factor and interleukin-6 are produced by diseased and regenerating skeletal muscle. Muscle & Nerve. ,vol. 19, pp. 1291- 1301 ,(1996) , 10.1002/(SICI)1097-4598(199610)19:10<1291::AID-MUS6>3.0.CO;2-9
Joseph J. Crisco, Peter Jokl, Gregory T. Heinen, Marc D. Connell, Manohar M. Panjabi, A Muscle Contusion Injury Model Biomechanics, Physiology, and Histology American Journal of Sports Medicine. ,vol. 22, pp. 702- 710 ,(1994) , 10.1177/036354659402200521
T. M. Mayhew, M. A. Williams, The mass and size of normal and activated macrophages—Studies with a scanning interferometer Experientia. ,vol. 29, pp. 80- 81 ,(1973) , 10.1007/BF01913265