Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family.

摘要: Mouse Ly-6 proteins are characterized by lineage-restricted patterns of expression on lymphoid cells. A mAb (1A8) was produced to Ly-6G, a newly described member the locus. Based selective reactivity cloned gene products expressed in EL4J cells, 1A8 determined be specific for Ly-6G. Furthermore, other specificities did not bind Ly-6G-transfected indicating that Ly-6G is distinct from serologically defined specificities. FACS analysis using demonstrated bone marrow but substantially tissues, including activated T and B In marrow, primarily restricted cells with more forward angle light scatter, which mostly granulocytes. The RB6-8C5 mAb, previously detect myeloid-restricted Ag (Gr-1) differentiated granulocytes, also reacted Ly-6G- Ly-6C-transfected Both selectively precipitate M(r) 21 25 kDa, glycosylphosphatidylinositol-anchored protein. Collectively, these data indicate Gr-1 family further link individual genes lineages mouse