KNOX1 is expressed and epigenetically regulated during in vitro conditions in Agave spp

摘要: The micropropagation is a powerful tool to scale up plants of economical and agronomical importance, enhancing crop productivity. However, small but growing body evidence suggests that epigenetic mechanisms, such as DNA methylation histone modifications, can be affected under the in vitro conditions characteristic micropropagation. Here, we tested whether adaptation different systems (Magenta boxes Bioreactors) modified epigenetically clones Agave fourcroydes A. angustifolia. Furthermore, assessed these changes affect regulatory expression KNOTTED1-like HOMEOBOX (KNOX) transcription factors. To gain better understanding during ex angustifolia, analyzed global methylation, well modification marks, two systems: semisolid Magenta (M) temporary immersion modular Bioreactors (B). No significant difference was found grown either M or B. when compared with there two-fold between species, independent system used. detected an absence low amount repressive mark H3K9me2 were cultured earlier Moreover, AtqKNOX1 AtqKNOX2, on angustifolia clones, conditions. Therefore, used Chromatin ImmunoPrecipitation (ChIP) know genes regulated. In case AtqKNOX1, H3K4me3 comparison AtqKNOX2. higher adapted addition, displayed differential KNOX1 gene conditions, which regulated by marks. finding regulation key developmental will make it important future studies identify factors help find climate-resistant micropropagated plants.