Plasma Progesterone Concentration after Removal of the Corpus Luteum in the Cow

摘要: PLASMA progesterone concentration was determined in serial 1-l. blood samples taken from normal dairy cows at mid-cycle after removal of the corpus luteum. The method Short1, with some modification, notably incorporation an internal marker progesterone-4-14C for individual recoveries, used determination. In addition it found necessary to acetylate first chromatogram eluate and subject rechromatography2,3. Recoveries were comparable those obtained bovine plasma by Short1 averaged 50 per cent a range 40–60 cent. Corpora lutea removed under posterior epidural anaesthesia via vagina making 3-cm incision, dorsal vaginal fornix, manipulating ovaries into where luteum easily enucleated. this position could be inspected means speculum either residual tissue left enucleation or other corpora lutea.