Mouse Sperm Lacking ADAM1b/ADAM2 Fertilin Can Fuse with the Egg Plasma Membrane and Effect Fertilization
作者： Ekyune Kim , Misuzu Yamashita , Tomoko Nakanishi , Ki-Eun Park , Masanori Kimura
关键词: Oviduct 、 Uterus 、 ADAM3 、 Human fertilization 、 Sperm 、 Mutant 、 Cell 、 Immunology 、 Cell biology 、 Zona pellucida 、 Biology 、 Biochemistry 、 Molecular biology
摘要: Fertilin, a heterodimeric protein complex composed of α (ADAM1) and β (ADAM2) subunits on the sperm surface, is believed to mediate adhesion fusion between egg plasma membranes. Here we have shown that mutant male mice lacking ADAM1b are fertile loss results in no significant defect functions such as migration from uterus into oviduct, binding zona pellucida, with pellucida-free eggs. ADAM1b-deficient epididymal showed severe reduction ADAM2 cell despite normal presence testicular germ cells. The appearance surface depended formation abundance ADAM1b/ADAM2 fertilin These suggest mouse may play crucial role not sperm/egg but these two ADAMs surface.
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