Distinction between epidermal antigens binding pemphigus vulgaris and pemphigus foliaceus autoantibodies.

摘要: Pemphigus vulgaris (PV) and pemphigus foliaceus (PF) are autoimmune blistering diseases in which antibodies develop to the cell surface of epidermal cells. In this study we sought determine antigenic specificity sera patients with PV PF. Sera from 12 were used immunoprecipitate extracts cultured human cells that radiolabeled 14C-amino acids. Immunoprecipitates identified by SDS polyacrylamide gel electrophoresis (PAGE) fluorography. All precipitated a protein which, when reduced, displayed chains 130,000 80,000 mol wt on SDS-PAGE. Electrophoresis under nonreducing conditions 210,000-mol molecule, was presumably formed disulfide crosslinking 80,000-mol chains. labeled 14C-glucosamine indicated 130,000-mol chain. Seven eight PF sera, run concurrently immunoprecipitation assay, did not precipitate glycoprotein, nor they specifically any protein. To if specific molecule reacted could be identified, immunoblot analysis normal epidermis. The proteins these separated SDS-PAGE, electrophoretically transferred nitrocellulose sheets or 2-aminophenylthioether paper. Immunoperoxidase staining four contained stained band 160,000 wt. Indirect immunofluorescence, using skin as substrate, IgG eluted epidermis pattern. recognize bands analysis. Immunoblots performed antigen immunoprecipitated culture suggested that, once denatured for is no longer immunoreactive. Taken together, data indicate that: autoantibodies various have unique molecular specificity; most different autoantibodies; may identical specificities. These differences between account clinical histologic diseases.