Electrochemical impedance immunosensor based on gold nanoparticles-protein G for the detection of cancer marker epidermal growth factor receptor in human plasma and brain tissue.
作者： Reda Elshafey , Ana C. Tavares , Mohamed Siaj , Mohammed Zourob
DOI: 10.1016/J.BIOS.2013.05.063
关键词: Protein G 、 Epidermal growth factor receptor 、 Immunoassay 、 Nanoparticle 、 Detection limit 、 Chemistry 、 Molecular biology 、 Platelet-derived growth factor receptor 、 Selectivity 、 Colloidal gold
摘要: A sensitive label-free impedimetric immunosensor for the detection of cancer biomarker epidermal growth factor receptor (EGFR) was developed with a limit of detection as low as 0.34 pg mL− 1 in PBS and 0.88 pg mL− 1 in human plasma. The gold nanoparticles were electrodeposited to modify the gold surface and to increase the electrochemical active area by a factor of approximately 3, ie by 68%. Protein G was used as scaffold for well oriented EGFR antibodies immobilization. Under optimal experimental parameters, the impedance …
elsevier.com
sci-hub.se 参考文章(39)
Carolyn I. Sartor, Biological modifiers as potential radiosensitizers: targeting the epidermal growth factor receptor family. Seminars in Oncology. ,vol. 27, pp. 15- 20 ,(2000)
Neale Ridgway, Zhaolin Xu, Drew Bethune, Gillian Bethune, Epidermal growth factor receptor (EGFR) in lung cancer: an overview and update Journal of Thoracic Disease. ,vol. 2, pp. 48- 51 ,(2010) , 10.3978/J.ISSN.2072-1439.2010.02.01.017
Erhan Dinçkaya, Özer Kınık, Mustafa Kemal Sezgintürk, Çağrı Altuğ, Aylin Akkoca, Development of an impedimetric aflatoxin M1 biosensor based on a DNA probe and gold nanoparticles. Biosensors and Bioelectronics. ,vol. 26, pp. 3806- 3811 ,(2011) , 10.1016/J.BIOS.2011.02.038
Shufeng Liu, Li Wang, Feng Zhao, Influence of gold nanoparticle modified electrode on the mediation reduction of ferricyanide by methylene blue Journal of Electroanalytical Chemistry. ,vol. 602, pp. 55- 60 ,(2007) , 10.1016/J.JELECHEM.2006.11.029
P Pfeiffer, E Nexø, SM Bentzen, PP Clausen, K Andersen, C Rose, Enzyme-linked immunosorbent assay of epidermal growth factor receptor in lung cancer: comparisons with immunohistochemistry, clinicopathological features and prognosis British Journal of Cancer. ,vol. 78, pp. 96- 99 ,(1998) , 10.1038/BJC.1998.448
A. Kausaite-Minkstimiene, A. Ramanaviciene, J. Kirlyte, A. Ramanavicius, Comparative Study of Random and Oriented Antibody Immobilization Techniques on the Binding Capacity of Immunosensor Analytical Chemistry. ,vol. 82, pp. 6401- 6408 ,(2010) , 10.1021/AC100468K
Renjun Pei, Zhiliang Cheng, Erkang Wang, Xiurong Yang, Amplification of antigen–antibody interactions based on biotin labeled protein–streptavidin network complex using impedance spectroscopy Biosensors and Bioelectronics. ,vol. 16, pp. 355- 361 ,(2001) , 10.1016/S0956-5663(01)00150-6
Ali A. Ensafi, M. Taei, H.R. Rahmani, T. Khayamian, Sensitive DNA impedance biosensor for detection of cancer, chronic lymphocytic leukemia, based on gold nanoparticles/gold modified electrode Electrochimica Acta. ,vol. 56, pp. 8176- 8183 ,(2011) , 10.1016/J.ELECTACTA.2011.05.124
Shabnam Siddiqui, Zhenting Dai, Courtney J. Stavis, Hongjun Zeng, Nicolaie Moldovan, Robert J. Hamers, John A. Carlisle, Prabhu U. Arumugam, A quantitative study of detection mechanism of a label-free impedance biosensor using ultrananocrystalline diamond microelectrode array Biosensors and Bioelectronics. ,vol. 35, pp. 284- 290 ,(2012) , 10.1016/J.BIOS.2012.03.001
Jie Yu, David Javier, Mohammad A. Yaseen, Nitin Nitin, Rebecca Richards-Kortum, Bahman Anvari, Michael S. Wong, Self-assembly synthesis, tumor cell targeting, and photothermal capabilities of antibody-coated indocyanine green nanocapsules. Journal of the American Chemical Society. ,vol. 132, pp. 1929- 1938 ,(2010) , 10.1021/JA908139Y