Development of an impedimetric aflatoxin M1 biosensor based on a DNA probe and gold nanoparticles.
作者： Erhan Dinçkaya , Özer Kınık , Mustafa Kemal Sezgintürk , Çağrı Altuğ , Aylin Akkoca
DOI: 10.1016/J.BIOS.2011.02.038
关键词:
摘要: The present work describes the construction and application of a new DNA biosensor for detection aflatoxin M1. In order to immobilize thiol-modified single stranded (ss-HSDNA) probe that specifically bound M1, self-assembled monolayer cysteamine gold nanoparticles on SAM were prepared electrodes, layer-by-layer. assembly processes cysteamine, nanoparticles, ss-HSDNA monitored with help electrochemical impedance spectroscopy (EIS) cyclic voltammetry (CV) techniques. K3[Fe(CN)6]/K4[Fe(CN)6] solution was used as redox measurements. provided linear response M1 over concentration range 1-14 ng/mL standard deviation ±0.36 ng/mL. Finally, applied series real milk samples.
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sci-hub.se 参考文章(37)
Richard J. Cole, Modern methods in the analysis and structural elucidation of mycotoxins. Academic Press, Inc.. ,(1986)
S.S. Deshpande, Handbook of Food Toxicology ,(2002)
F. S. Chu, Immunoassays for mycotoxins: current state of the art, commercial and epidemiological applications. Veterinary and Human Toxicology. ,vol. 32, pp. 42- 50 ,(1990)
Hans P. van Egmond, Ronald C. Schothorst, Marco A. Jonker, Regulations relating to mycotoxins in food Analytical and Bioanalytical Chemistry. ,vol. 389, pp. 147- 157 ,(2007) , 10.1007/S00216-007-1317-9
Shipra Rastogi, Premendra D. Dwivedi, Subhash K. Khanna, Mukul Das, Detection of Aflatoxin M1 contamination in milk and infant milk products from Indian markets by ELISA Food Control. ,vol. 15, pp. 287- 290 ,(2004) , 10.1016/S0956-7135(03)00078-1
H.P. van Egmond, Mycotoxins in dairy products Food Chemistry. ,vol. 11, pp. 289- 307 ,(1983) , 10.1016/0308-8146(83)90076-6
Toshikazu Shibahara, H.Iyehara Ogawa, Haruko Ryo, Kazuo Fujikawa, DNA-damaging potency and genotoxicity of aflation M1 in somatic cells in vivo of Drosophila melanogaster Mutagenesis. ,vol. 10, pp. 161- 164 ,(1995) , 10.1093/MUTAGE/10.3.161
S.R. Garden, N.J.C. Strachan, Novel colorimetric immunoassay for the detection of aflatoxin B1 Analytica Chimica Acta. ,vol. 444, pp. 187- 191 ,(2001) , 10.1016/S0003-2670(01)01231-4
John L. Richard, Some major mycotoxins and their mycotoxicoses--an overview. International Journal of Food Microbiology. ,vol. 119, pp. 3- 10 ,(2007) , 10.1016/J.IJFOODMICRO.2007.07.019
C Lopez, L Ramos, S Ramadan, L Bulacio, J Perez, None, Distribution of aflatoxin M1 in cheese obtained from milk artificially contaminated International Journal of Food Microbiology. ,vol. 64, pp. 211- 215 ,(2001) , 10.1016/S0168-1605(00)00444-X