Isolation, Culture Characteristics, and Identification of Anaerobic Bacteria from the Chicken Cecum

摘要: Studies on the anaerobic cecal microflora of 5-week-old chicken were made to determine a suitable roll-tube medium for enumeration and isolation bacterial population, effects components recovery total anaerobes, identify predominant groups. The number microorganisms in contents determined by direct microscope cell counts varied (among six samples) from 3.83 × 1010 7.64 per g. Comparison different nonselective media indicated that 60% count could be recovered with rumen fluid (M98-5) 45% 10. Deletion M98-5 reduced half. Colony lower if extract was substituted M98-5. Supplementing 10 liver, fecal, or extracts improved anaerobes slightly. Prereduced blood agar inferior At least 11 groups bacteria isolated high dilutions (10-9) material. Data morphology physiological fermentation characteristics 90% 298 strains these represented species gram-negative cocci, facultatively cocci streptococci, Peptostreptococcus, Propionibacterium, Eubacterium, Bacteroides, Clostridium. growth many enhanced fluid, yeast extract, additions basal media. These studies indicate some more numerous present digesta can cultured when methods have been developed ruminal are employed.